Identification and characterization of artery and vein enhancers in the human genome. Identification and characterization of artery and vein enhancers in the human genome

Our goal was to identify artery- and vein-specific cis regulatory elements in the human genome and use these to determine novel mechanisms that regulate arteriovenous differentiation Overall design: Artery and vein enhancers were identified using ChIP-Seq for K27ac and EP300 on HUAEC and HUVEC, performed in duplicate. Further analysis included RNAseq in triplicate from the same cell types. To assess binding of known transcription factors NR2F2 and ERG, we performed ChIP-Seq in duplicate samples. We assessed K27ac and K27me3 occupancy under different experimental conditions using triplicate samples from HUVEC

本研究旨在识别人类基因组中动脉与静脉特异性的顺式调控元件（cis regulatory elements），并依托这些元件阐明调控动静脉分化的全新机制。
实验整体设计：
通过针对人脐动脉内皮细胞（HUAEC, Human Umbilical Artery Endothelial Cells）与人脐静脉内皮细胞（HUVEC, Human Umbilical Vein Endothelial Cells）的K27ac及EP300的染色质免疫共沉淀测序（ChIP-Seq）鉴定动脉与静脉增强子，该实验设置两次生物学重复。
后续分析采用上述两种细胞的转录组测序（RNA-seq）样本，设置三次生物学重复。
为检测已知转录因子NR2F2与ERG的结合情况，我们对样本进行了两次生物学重复的ChIP-Seq实验。
我们采用来自HUVEC的三次生物学重复样本，检测了不同实验条件下K27ac与K27me3的结合占据水平。