Identification of 5-methylcytosine associated genes in osteoarthritis

Our study aimed to identify common m5C features in OA. We identified we found 35828 m5C peaks and mapped up to 11720 annotated genes in OA samples, and we found 16259 m5C peaks and mapped up to 7504 annotated genes in normal samples, indicating an overall hypermethylated level in the OA cartilage. Overall design: We collected three OA knee cartilage tissues and three normal knee cartilage tissues to obtain the transcriptome-wide m5C modification profile of genes in OA by methylated RNA immunoprecipitation next-generation sequencing (MeRIP-seq).

本研究旨在识别骨关节炎（Osteoarthritis, OA）中常见的5-甲基胞嘧啶（5-methylcytosine, m5C）修饰特征。本研究在骨关节炎样本中共鉴定得到35828个m5C峰，并比对到至多11720个已注释基因；在正常样本中则鉴定得到16259个m5C峰，比对到至多7504个已注释基因，提示骨关节炎软骨整体呈现高甲基化修饰水平。实验设计：本研究采集3份骨关节炎膝关节软骨组织与3份正常膝关节软骨组织，通过甲基化RNA免疫沉淀下一代测序（methylated RNA immunoprecipitation next-generation sequencing, MeRIP-seq）获取骨关节炎样本中基因的全转录组m5C修饰图谱。