Native plants in which infection with introduced or unidentified viruses was detected by ELISA tests on samples

For an explanation of virus acronyms see Table 1,- = Not tested,* = TuYV detected by BWYV polyclonal antibodies.** = Tospovirus positive sample tested negative for TSWV and Impatiens necrotic spot virus (INSV),+ = Native orchid collection. Samples were either tested individually or grouped (in 5’s-10’s) before testing. When sufficient grouped samples were present, percentage infection was calculated using the formula of Gibbs and Gower [71]. All orchid samples also tested for Cymbidium mosaic virus and Odontoglossum ringspot virus by ELISA, but none contained them.

病毒缩写的详细说明详见表1。- 代表未检测；* 代表通过甜菜西方黄化病毒（Beet Western Yellows Virus, BWYV）多克隆抗体检测出芜菁黄化病毒（Turnip Yellows Virus, TuYV）；** 代表该样品为番茄斑萎病毒属（Tospovirus）阳性，但对番茄斑点萎蔫病毒（Tomato Spotted Wilt Virus, TSWV）与凤仙花坏死斑病毒（Impatiens Necrotic Spot Virus, INSV）的检测结果呈阴性；+ 代表原生兰花采集样品。样品可单独开展检测，或按5~10份为一组混合后再进行检测。当混合样品的样本量足够时，感染率按照Gibbs与Gower[71]提出的公式进行计算。所有兰花样品均通过酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay, ELISA）检测了建兰花叶病毒（Cymbidium Mosaic Virus）与齿舌兰环斑病毒（Odontoglossum Ringspot Virus），但未检出上述两种病毒。