Permutational analysis of Saccharomyces cerevisiae regulatory elements

Gene expression in Saccharomyces cerevisiae is regulated at multiple levels. Genomic and epigenomic mapping of transcription factors and chromatin factors has led to the delineation of various modular regulatory elements, Enhancers, core promoters, 5 prime untranslated regions and 3 prime untranslated regions and transcriptional terminators. We describe a simple and rapid approach to build a combinatoric library of regulatory elements and have used this library to study expression of components from 26 genes.

酿酒酵母（Saccharomyces cerevisiae）的基因表达在多个层面受到调控。针对转录因子与染色质因子的基因组及表观基因组图谱绘制研究，已阐明多种模块化调控元件，包括增强子（Enhancers）、核心启动子（core promoters）、5'非翻译区（5 prime untranslated regions）、3'非翻译区（3 prime untranslated regions）以及转录终止子（transcriptional terminators）。本研究描述了一种构建调控元件组合文库的简便快速方法，并利用该文库对26个基因的组分表达进行了研究。