Transcription profiling of major depression peripheral blood mononuclear cells (PBMCs) at clinical remission compared to severe acute state

PBMCs from major depressive episode (MDE) subjects were obtained at two time points: during a severe episode of depression and eight week later after clinical remission. PBMCs from sex- and age-machted controls were also obtained at an eight weeks interval. PBMC total RNAs from MDE and control subjects were profiled after hybridization with Agilent SurePrint G3 Human GE 8x60K Microarray to identify blood biomarkers of MDE. Total RNAs were extracted from 36 samples including: 9 control and 9 MDE subjects at two point of time (first and eight week later)

本研究的样本来自重性抑郁发作（major depressive episode, MDE）受试者的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs），采集于两个时间节点：抑郁严重发作期及临床缓解8周后。同时还采集了性别与年龄匹配的健康对照者的外周血单个核细胞，同样以8周为间隔完成两次采样。随后，采用安捷伦SurePrint G3人类基因表达8×60K微阵列（Agilent SurePrint G3 Human GE 8x60K Microarray）对重性抑郁发作组与对照组的外周血单个核细胞总RNA进行芯片杂交分析，以筛选重性抑郁发作的血液生物标志物。本次研究共提取36份样本的总RNA，涵盖9名对照者与9名重性抑郁发作受试者的两个时间点（首次采样时及8周后）的样本。