The use of a new calcium mesoporous silica nanoparticle versus calcium and/or fluoride products in reducing the progression of dental erosion

Abstract Objective There is increasingly common the consumption more times a day of foods and acidic drinks in the diet of the population. The present study aimed to evaluate and compare the effects of a calcium mesoporous silica nanoparticle single application of other calcium and/or fluoride products in reducing the progression of dental erosion. Methodology Half of the eroded area was covered of 60 blocks of enamel, after which the block was submitted to the following treatments: (Ca2+-MSN), casein phosphopeptide–amorphous calcium phosphate (CPP-ACP); CPP-ACP/F-(900 ppm F−); titanium tetrafluoride (TiF4 1%) (positive control); sodium fluoride (NaF 1.36%) (positive control); and Milli-Q® water (negative control) before being submitted to a second erosive challenge. A surface analysis was performed via a three-dimensional (3D) noncontact optical profilometry to assess the volumetric roughness (Sa) and tooth structure loss (TSL) and and through scanning electron microscopy (MEV). An analysis of variance (ANOVA) and Tukey’s test were performed. Results Regarding Sa, all experimental groups exhibited less roughness than the control (p<0.05). The TSL analysis revealed that the Ca2+-MSN and NaF groups were similar (p>0.05) and more effective in minimizing tooth loss compared with the other groups (p<0.05). Conclusions The Ca2+-MSN and NaF treatments were superior compared with the others and the negative control.

【摘要】 研究目的：目前人群日常饮食中，每日多次摄入酸性食物与饮料的情况愈发普遍。本研究旨在评估并比较单次应用介孔硅酸钙纳米颗粒（calcium mesoporous silica nanoparticle, Ca²⁺-MSN）与其他含钙、含氟制剂，在抑制牙釉质侵蚀进展方面的效果。

方法：本研究制备60颗牙釉质块，将每块的侵蚀区域覆盖一半后，分别接受以下处理：介孔硅酸钙纳米颗粒（Ca²⁺-MSN）组、酪蛋白磷酸肽-无定形磷酸钙（casein phosphopeptide–amorphous calcium phosphate, CPP-ACP）组、CPP-ACP/氟（含900 ppm F⁻）组、四氟化钛（titanium tetrafluoride, TiF₄ 1%）阳性对照组、氟化钠（sodium fluoride, NaF 1.36%）阳性对照组，以及Milli-Q®纯水阴性对照组，随后再次开展侵蚀性攻击实验。采用三维（3D）非接触式光学轮廓仪进行表面分析，以评估体积粗糙度（Sa）与牙体结构损失量（TSL），并通过扫描电子显微镜（原文缩写为MEV）完成观测；同时采用方差分析（analysis of variance, ANOVA）与Tukey检验进行统计学分析。

结果：针对体积粗糙度Sa，所有实验组的粗糙度均低于对照组（p<0.05）。牙体结构损失量TSL分析结果显示，介孔硅酸钙纳米颗粒组与氟化钠组的防牙体丢失效果相当（p>0.05），且均优于其余各组（p<0.05）。

结论：介孔硅酸钙纳米颗粒与氟化钠的处理效果均优于其余实验组及阴性对照组。