Phosphorescence monitoring of hypoxic microenvironment in solid-tumors to evaluate chemotherapeutic effects using the hypoxia-sensitive iridium (III) coordination compound

Objectives: To utilize phosphorescence to monitor hypoxic microenvironment in solid-tumors and investigate cancer chemotherapeutic effects in vivo. Methods: A hypoxia-sensitive probe named BTP was used to monitor hypoxic microenvironment in solid-tumors. The low-dose metronomic treatment with cisplatin was used in anti-angiogenetic chemotherapeutic programs. The phosphorescence properties of BTP were detected by a spectrofluorometer. BTP cytotoxicity utilized cell necrosis and apoptosis, which were evaluated by trypan blue dye exclusion and Hoechst33342 plus propidium iodide assays. Tumor-bearing mouse models of colon adenocarcinoma were used for tumor imaging in vivo. Monitoring of the hypoxic microenvironment in tumors was performed with a Maestro 2 fluorescence imaging system. Tumor tissues in each group were harvested regularly and treated with pathological hematoxylin and eosin and immunohistochemical staining to confirm imaging results. Results: BTP did not feature obvious cytotox...

研究目的：利用磷光技术监测实体瘤的缺氧微环境，并在体内探究癌症的化疗效果。
研究方法：采用一种命名为BTP的缺氧敏感探针，对实体瘤的缺氧微环境进行监测；在抗血管生成化疗方案中，采用顺铂低剂量节律给药方式；通过荧光分光光度计检测BTP的磷光特性；通过细胞坏死与凋亡评估BTP的细胞毒性，分别采用台盼蓝拒染法与Hoechst33342联合碘化丙啶（propidium iodide）实验进行检测；构建结肠腺癌荷瘤小鼠模型用于体内肿瘤成像；采用Maestro 2荧光成像系统对肿瘤内的缺氧微环境进行监测；定期采集各组肿瘤组织，进行病理苏木精-伊红（hematoxylin and eosin）染色与免疫组化染色，以验证成像结果。
研究结果：BTP未表现出明显的细胞毒……