A novel zebrafish model of intestinal epithelial damage reveals macrophages and igfbp1a as major modulators of mucosal healing

To elucidate the cellular and molecular mechanisms governing intestinal damage and regeneration in zebrafish, we performed single-cell (sc)RNA-seq from dissected intestines. Intestines from control and Mtz-treated zebrafish larvae were dissected at 4 days post fertilization (dpf) and 5dpf. Single cell suspensions from dissected intestines were prepared througn enzymatic and mechanical disaggregation, and DAPI- live cells were sorted by FACS.

为阐明斑马鱼肠道损伤与再生的细胞及分子调控机制，本研究对解剖获取的肠道组织开展了单细胞RNA测序（single-cell RNA-seq，scRNA-seq）。我们于受精后4天（days post fertilization，dpf）及5天（dpf），分别解剖获取对照组与甲硝唑（Mtz）处理的斑马鱼幼鱼的肠道组织。通过酶解与机械解离的方法制备上述肠道组织的单细胞悬液，并借助荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分选出DAPI阴性的活细胞。