Ghrelin treatment restores alpha-diversity of the colonic microbiome of bile duct-ligated mice

C57BL/6J mice with bile duct ligation (BDL) or sham surgery were treated with ghrelin or solvent for 9 days. Amplicon metagenomic sequencing was employed to analyze bacterial 16S rRNA from colonic stool samples.Reduced alpha-diversity of the microbiome was observed in solvent-treated mice with BDL but not in ghrelin-treated animals.

对接受胆管结扎（bile duct ligation, BDL）或假手术的C57BL/6J小鼠，分别予以生长激素释放肽（ghrelin）或溶剂处理，干预时长为9天。本研究采用扩增子宏基因组测序（amplicon metagenomic sequencing）分析结肠粪便样本中的细菌16S rRNA。结果显示，仅在接受溶剂处理的BDL小鼠中观察到微生物组α多样性降低，而生长激素释放肽处理的受试动物未出现该现象。