16s pyrosequencing on Ossekampen grassland soil

The relationship between specific environmental factors, microbial community structures and functions is still poorly investigated. Here, we chose samples from a 54- year fertilized (N-, P-, K- and NPK- amended, including non-fertilized) field and examined 29 suits of soil chemical and physical parameters and applied next generation sequencing for 16S rRNA gene fragment and DNA shotgun-metagenomic approaches to analyze the diversity and functional characteristics of soil microbial community. Soil physicochemical parameters differed significantly among different treatments. Total community compositions shifted between different fertilizers. However, there was no fertilizer effect on the general functional profiles. Different soil parameters had significant correlations with different microbial functions. Overall, our results demonstrated functional redundancy in microbial community in soil and suggested that more detailed soil physicochemical parameters rather than pH need to be investigated. Keywords: metagenomics/ microbial community functions / microbial community structures/ environmental factors

特定环境因子（environmental factors）、微生物群落结构（microbial community structures）与微生物群落功能（microbial community functions）之间的关联，目前仍鲜有深入探究。本研究选取一块历经54年施肥的农田土壤样品，设置的处理包括不施肥对照、单施氮肥、单施磷肥、单施钾肥以及氮磷钾配施；研究中测定了29项土壤理化参数，借助下一代测序技术（Next Generation Sequencing）对16S rRNA基因片段（16S rRNA gene fragment）进行测序，并采用DNA鸟枪宏基因组学（DNA shotgun-metagenomic）方法，解析土壤微生物群落的多样性与功能特征。结果显示，不同施肥处理间的土壤理化参数存在显著差异，整体微生物群落组成随施肥类型的不同发生显著演替，但施肥处理对微生物群落的整体功能谱并无显著影响。此外，不同土壤参数与特定微生物功能之间存在显著相关性。综上，本研究结果揭示了土壤微生物群落存在功能冗余（functional redundancy）现象，并提示未来研究应聚焦于更精细的土壤理化参数分析，而非仅关注pH值。关键词：宏基因组学 / 微生物群落功能 / 微生物群落结构 / 环境因子