Data from: Coronavirus M protein disperses the trans-Golgi network and inhibits anterograde protein trafficking in the secretory pathway

This dataset contains all biological replicates for immunoblots and
immunofluorescence microscopy images associated with the manuscript
"Coronavirus M protein disperses the trans-Golgi network and inhibits
anterograde protein trafficking in the secretory pathway". These
figures demonstrate that the SARS-CoV-2 M protein inhibits the ATF6 branch
of the unfolded protein response but not the PERK or IRE1 branches.
Additionally, the SARS-CoV-2 M protein localizes to the cis-Golgi while
leading to dispersal of the trans-Golgi network and preventing anterograde
trafficking of host proteins beyond the cis-Golgi. Furthermore, SARS-CoV-2
M recruits cholesterol to this site. This data provides a greater
understanding of how SARS-CoV-2 M restructures the host secretory pathway
which consequentially inhibits host protein trafficking and secretion,
outlining a novel role for coronavirus M proteins beyond assembly and
structure.

本数据集包含与学术论文《冠状病毒M蛋白分散反式高尔基体网络（trans-Golgi network）并抑制分泌通路中的顺向蛋白转运》相关的全部免疫印迹实验（immunoblots）与免疫荧光显微镜成像（immunofluorescence microscopy）的生物学重复样本。上述实验结果证实，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的M蛋白可抑制未折叠蛋白反应（unfolded protein response）的ATF6通路，而非PERK或IRE1通路。此外，SARS-CoV-2 M蛋白定位于顺式高尔基体（cis-Golgi），同时引发反式高尔基体网络的分散，并阻断宿主蛋白在顺式高尔基体之后的顺向蛋白转运。进一步研究显示，SARS-CoV-2 M蛋白可将胆固醇招募至该作用位点。本数据集有助于深入阐明SARS-CoV-2 M蛋白重塑宿主分泌通路的分子机制，进而抑制宿主蛋白的转运与分泌，明确了冠状病毒M蛋白在病毒组装与结构功能之外的全新生物学作用。