Differential Expression of LncRNAs and mRNAs in normal and inflamed human pulp. Homo sapiens

Dental pulp inflammation provides an excellent model for the interaction between tissue inflammation and regenerative processes. It is worthwhile to better understand molecular signaling of repair and regeneration in inflammatory processes. Emerging evidence suggests that lncRNAs participate in immune/inflammatory processes. We aimed to investigate the expression of lncRNAs in pulpitis and predict lncRNAs that possibly involved in inflammation and odontogenesis. Overall design: For microarray analysis, seven cases of inflamed pulp tissue samples and five cases of normal pulp tissue samples were obtained.Normal pulps were picked from healthy third molars or teeth extracted for orthodontic purpose.Inflamed pulp tissues were extracted from teeth diagnosed with irreversible pulpitis in accordance with the endodontics diagnoses system from the American Association of Endodontists. Any teeth that suffered from periodontitis were excluded. Patients who had a compromised immune system or those who were taking medications known to influence the immune response were excluded from the study.

牙髓炎症是研究组织炎症与再生过程相互作用的优质模型。深入解析炎症过程中修复与再生的分子信号通路具有重要研究价值。越来越多的研究证据表明，长链非编码RNA（long non-coding RNA, lncRNA）参与免疫与炎症过程。本研究旨在探究牙髓炎组织中lncRNA的表达情况，并预测可能参与炎症反应与牙发生过程的lncRNA。实验整体设计：本研究通过基因芯片分析，共获取7例炎症牙髓组织样本与5例正常牙髓组织样本。正常牙髓组织取自健康第三磨牙或因正畸治疗拔除的牙齿。炎症牙髓组织取自符合美国牙髓病学协会（American Association of Endodontists）牙髓病诊断标准的不可复性牙髓炎患牙。合并牙周炎的患牙均被排除。免疫系统功能受损或服用已知影响免疫应答药物的患者均被排除出本研究。