Data from: A field-deployable eDNA metabarcoding workflow including de novo reference assembly for characterizing understudied biodiversity hotspots

This dataset contains the data underlying the manuscript A field-deployable eDNA metabarcoding workflow including de novo reference assembly for characterizing understudied biodiversity hotspotsJesse Erens, Christopher Heine, Stefan Lötters, Henrik Krehenwinkel, Andrew J. Crawford, Luis Alberto Rueda-Solano, Amadeus Plewnia--- The .fastq files contain the demultiplexed raw reads from all samples (eDNA and single-specimen samples) as basecalled with Dorado 0.9.0 and demultiplexed using MiniBar (Krehenwinkel et al. 2019, doi: 10.1093/gigascience/giz006).--- Read count tables show the number of reads assigned to each taxon, with columns corresponding to each individual sampling site (first number), field replicate (second number) and RPA replicate (third number), following the site acronyms as outlined in the Supplementary Material of the manuscript. Processing of reads as described in the original manuscript (based on Plewnia et al. 2025, doi: 10.1111/2041-210X.70069).

本数据集包含支撑下述学术论文的相关数据：题为《可野外部署的环境DNA（eDNA）宏条形码（metabarcoding）工作流及从头参考序列组装》，作者为Jesse Erens、Christopher Heine、Stefan Lötters、Henrik Krehenwinkel、Andrew J. Crawford、Luis Alberto Rueda-Solano及Amadeus Plewnia。

.fastq格式文件包含所有样本（环境DNA样本及单标本样本）的解复用原始测序读段（reads），经Dorado 0.9.0完成碱基识别，并通过MiniBar（Krehenwinkel等，2019，doi: 10.1093/gigascience/giz006）完成解复用。

测序读段计数表展示了分配至各分类单元的读段数量，表格列依次对应单个采样位点（第一位数字）、野外重复（第二位数字）及RPA重复（第三位数字），采样位点缩写规则详见论文补充材料。

测序读段的处理流程详见原论文（基于Plewnia等，2025，doi: 10.1111/2041-210X.70069）。