Effect of FL-Fc-DM1 drug on gene expression of THP-1 cells

To eliminate cell-cycle arrested AML cells, we developed a FL-Fc-DM1 drug by conjugating FLT3 liangd Fc fusion protein and mertansine. We used the THP-1 cells as a AML model. The total mRNA of THP-1 cells treated with different concentration of FL-Fc-DM1 were sequenced. THP-1 cells were cultured in RPMI 1640 medium supplemented with 10% FBS. In the control group, THP-1 cells were cultured for 1 day. In the FL-Fc-DM1 treatment groups, THP-1 cells were treated with FL-Fc-DM1 (1 and 3 nM) for 24 hours. We than analyze the transcriptome differences between different groups.

为清除细胞周期阻滞的急性髓系白血病（AML）细胞，我们通过将FLT3配体（FLT3 ligand）-Fc融合蛋白与美登木素（mertansine）偶联，开发了FL-Fc-DM1药物。我们选用THP-1细胞作为AML细胞模型。THP-1细胞培养于添加10%胎牛血清（FBS）的RPMI 1640培养基中。对照组中，THP-1细胞仅培养1天；FL-Fc-DM1处理组中，THP-1细胞分别以1 nM和3 nM浓度的FL-Fc-DM1处理24小时。对经不同浓度FL-Fc-DM1处理的THP-1细胞进行总mRNA测序，随后分析各组之间的转录组差异。