Dynamics of the rangpur lime seed germination test conducted under different temperatures

Abstract: Adoption of a standard procedure to perform the germination test allows comparison of results between laboratories. Thus, the aim of this study was to evaluate the germination process of rangpur lime (Citrus limonia Osbeck) seeds conducted under different temperatures. The germination test was carried out in five seed lots under three temperatures (25, 30 and 35 °C). The dynamics of the germination process was studied through the following variables: germination (%), time to first germination (days), time to last germination (days), time interval between first and last germination (days), time required to reach 50% of germination (days), mean speed of germination (day-1), synchrony of the germination process (dimensionless), and uncertainty of the germination process (bits). The experimental design was completely randomized, and analysis of variance was performed in a 5 (lots) x 3 (temperatures) factorial arrangement, with four replicates of each treatment. Means were compared using Tukey’s test (p < 0.05). The germination process in rangpur lime seeds is slow, homogenous, and asynchronous; and the temperature of 30 °C is the most favorable for conducting the germination test, with the first count at 21 days and the last at 30 days.

摘要：采用标准化流程开展发芽试验，可实现不同实验室间试验结果的有效比对。本研究旨在评估不同温度条件下黎檬（Citrus limonia Osbeck）种子的发芽过程。试验选取5个种子批，设置25、30、35 ℃三种温度梯度开展发芽试验。通过以下指标分析发芽过程的动态变化：发芽率（%）、首次发芽天数、末次发芽天数、首尾发芽间隔天数、达到50%发芽率所需天数、平均发芽速率（d⁻¹）、发芽同步性（无量纲）以及发芽过程不确定性（bits）。本试验采用完全随机设计，以5（种子批）×3（温度）的析因安排设置处理，每个处理设置4次重复。采用Tukey检验进行均值比较（p < 0.05）。结果表明，黎檬种子发芽过程缓慢、均一性差且异步性显著；30 ℃为最适宜的发芽试验温度，首次计数时间为21天，末次计数时间为30天。