Table_3_Identification of Gene Expression Changes Associated With Uterine Receptivity in Mice.XLSX

The mouse is a widely used animal model for studying human reproduction. Although global gene expression changes associated with human uterine receptivity have been determined by independent groups, the same studies in the mouse are scarce. The extent of similarities/differences between mice and humans on uterine receptivity at the molecular level remains to be determined. In the present study, we analyzed global gene expression changes in receptive uterus on day 4 of pregnancy compared to non-receptive uterus on day 3 of pregnancy in mice. A total of 541 differentially expressed genes were identified, of which 316 genes were up-regulated and 225 genes were down-regulated in receptive uterus compared to non-receptive uterus. Gene ontology and gene network analysis highlighted the activation of inflammatory response in the receptive uterus. By analyzing the promoter sequences of differentially expressed genes, we identified 12 causal transcription factors. Through connectivity map (CMap) analysis, we revealed several compounds with potential anti-receptivity activity. Finally, we performed a cross-species comparison against human uterine receptivity from a published dataset. Our study provides a valuable resource for understanding the molecular mechanism underlying uterine receptivity in mice.

小鼠是研究人类生殖的常用动物模型。尽管多个独立研究团队已明确了与人类子宫容受性（uterine receptivity）相关的全局基因表达变化，但针对小鼠的同类研究仍较为匮乏。小鼠与人类在子宫容受性层面的分子水平异同程度尚待阐明。本研究中，我们对比分析了小鼠妊娠第4天的容受性子宫与妊娠第3天的非容受性子宫的全局基因表达变化。最终共鉴定出541个差异表达基因（differentially expressed genes），其中容受性子宫相较于非容受性子宫上调316个、下调225个。基因本体（Gene Ontology, GO）分析与基因网络分析揭示，容受性子宫中炎症应答通路被激活。通过对差异表达基因的启动子序列进行分析，我们鉴定出12个转录因子（transcription factors）。借助连接图谱（Connectivity Map, CMap）分析，我们筛选出多种具有潜在抗容受性活性的化合物。最后，我们结合已发表的人类子宫容受性数据集开展了跨物种比较分析。本研究为解析小鼠子宫容受性的分子机制提供了宝贵的研究资源。