Proteins with lower or higher expression upon depletion of TgNFS2 as found by label-free quantitative proteomics.

For each protein candidate (with www.ToxoDB.org and www.Uniprot.org identifier), log2 of the different ratio were calculated between the mean MaxQuant LFQ values (‘moyLFQ’) found for the TgISU1 (‘Mito’) and TgNFS2 (‘Apicoplast’) mutants, and the TATi ΔKu80 control (‘CTRL’). -log10(pvalue) is also provided. Putative subcellular localization was obtained from the hyperLOPIT data available on ToxoDB.org, or by manual annotation. CRISPR fitness score and transcriptomic data for tachyzoites (Tz) and bradyzoites (Bz) were obtained from ToxoDB.org. (XLSX)

针对每个带有ToxoDB数据库（www.ToxoDB.org）与UniProt数据库（www.Uniprot.org）标识符的蛋白候选物，我们计算了TgISU1（标记为"Mito"）与TgNFS2（标记为"Apicoplast"）突变体的MaxQuant无标记定量（Label-Free Quantification, LFQ）均值（命名为"moyLFQ"），以及TATi ΔKu80对照（标记为"CTRL"）之间差异比值的log₂转换值；同时提供了-log₁₀(p值)。推定的亚细胞定位信息取自ToxoDB.org公开的hyperLOPIT数据，或经人工注释获得。刚地弓形虫速殖子（Tachyzoites, Tz）与缓殖子（Bradyzoites, Bz）的CRISPR适配性评分及转录组数据均来自ToxoDB.org。本数据集采用XLSX格式存储。