Characterization of a Candida albicans Isolated from a recurrent Cervical Lymphadenitis Patient and it’s Clinic Indication. Characterization of a Candida albicans Isolated from a recurrent Cervical Lymphadenitis Patient and it’s Clinic Indication

Purpose: To reveal the global regulatory genes of clinical strain, we performed comparative transcriptomic analyses of the SC5314 and JL01.Methods: We isolated a clinical Candida albicans strain JL01 (Jinling Hospital in Nanjing, China) from a 21-year-old man who presented to our hospital with recurrent cervical lymphadenitis. Cells of the JL01 and SC5314 were inoculated from overnight cultures by 1% into liquid YPD plus 10% serum medium at 37°C for 3 h. Cells were collected and total RNA was extracted as described. RNA-Seq analysis was performed by the company Majorbio (Shanghai, China). mRNA was purified, fragmented and used to synthesize cDNA. The library products were sequenced on the HiSeq 4000 platform using the HisSeq 4000 PE Cluster Kit and HisSeq 4000 SBS Kit.Results: 421 genes were significantly differentially regulated (P≤0.05, |log2FC|≥1) in JL01 compared with SC5314. Of these 421 DEGs (differentially expressed genes), 216 were up-regulated, and 205 were down-regulated. Conclusions: Together the clinical, morphological and biological analyses, we suggest that azole drug resistance, invasive defect and hypo-virulence of the JL01 strain may correlate with its contribution in chronic fungal infection. Overall design: We isolated a clinical Candida albicans strain JL01 (Jinling Hospital in Nanjing, China) from a 21-year-old man who presented to our hospital with recurrent cervical lymphadenitis. Cells of the JL01 and SC5314 were inoculated from overnight cultures by 1% into liquid YPD plus 10% serum medium at 37°C for 3 h. Cells were collected and total RNA was extracted as described.

研究目的：为揭示临床菌株的全局调控基因，我们对SC5314与JL01菌株开展了比较转录组学分析。
研究方法：我们从中国南京金陵医院的一名因复发性颈淋巴结炎就诊于我院的21岁男性患者体内分离得到临床白色念珠菌（Candida albicans）菌株JL01。将JL01与SC5314的过夜培养物以1%的接种量接种至添加10%血清的液体YPD培养基中，于37℃培养3小时。收集细胞并按照既定方法提取总RNA。RNA测序（RNA-Seq）分析由中国上海的美吉生物医药（Majorbio）公司完成。对mRNA进行纯化、片段化并合成cDNA，随后利用HiSeq 4000平台，搭配HiSeq 4000 PE簇试剂盒与HiSeq 4000 SBS试剂盒对文库产物进行测序。
研究结果：相较于SC5314菌株，JL01菌株中共存在421个显著差异表达的基因（P≤0.05，|log₂FC|≥1）。在这421个差异表达基因（differentially expressed genes，简称DEGs）中，216个基因上调表达，205个基因下调表达。
研究结论：结合临床、形态学与生物学分析结果，我们认为JL01菌株的唑类药物耐药性、侵袭缺陷与低毒力，可能与其在慢性真菌感染中的致病贡献相关。
整体实验设计：我们从中国南京金陵医院的一名因复发性颈淋巴结炎就诊于我院的21岁男性患者体内分离得到临床白色念珠菌菌株JL01。将JL01与SC5314的过夜培养物以1%的接种量接种至添加10%血清的液体YPD培养基中，于37℃培养3小时。收集细胞并按照既定方法提取总RNA。