Human UniGene Set RZPD 1 - Zipf's Normalization

Five microarrays from a larger dataset used to demonstrate a normalization technique base on Zipf's law. The original data-set generated using a global genome-wide cDNA clone set (Human UniGene clone set RZPD 1 - http://www.rzpd.de - Build 138, NCBI), which consisted of ~33,792 cDNA clone inserts spotted in duplicate onto membranes (18). These microarrays (n=31) were hybridized with 33P-labeled cDNA derived from total RNA extracted from biopsy material from the sigmoidal colon of normal (control, n=11), and patients with Crohn's disease (condition A, n=10) and ulcerative colitis (condition B, n=10). Microarrays were probed according to established protocols and exposed to imaging plates overnight (BAS-MS 2325) and scanned at a 50 m resolution on a FLA-3000G phosphoimager (Raytest, Germany). Image gridding was carried out using VisualGrid® software (http://www.gpc-biotech.com). Keywords: other

本数据集从包含31张基因芯片的原始大型数据集中选取5张，用于演示基于齐夫定律（Zipf's law）的归一化技术。原始数据集采用全基因组互补DNA（complementary DNA, cDNA）克隆集构建，即人类UniGene克隆集RZPD 1（链接：http://www.rzpd.de，版本138，NCBI），该克隆集包含约33792个cDNA克隆插入片段，以重复点样的方式制备于膜片上(18)。本次研究所用的31张基因芯片，均与33P标记的cDNA进行杂交；该cDNA提取自正常对照（n=11）、克罗恩病患者（组别A，n=10）及溃疡性结肠炎患者（组别B，n=10）的乙状结肠活检组织的总RNA。基因芯片按照既定实验方案完成杂交后，置于成像板（BAS-MS 2325）中过夜曝光，随后使用FLA-3000G型磷光成像扫描仪（德国Raytest公司）以50 μm分辨率进行扫描。图像网格化操作采用VisualGrid®软件完成（链接：http://www.gpc-biotech.com）。关键词：其他