Expression data of neural progenitor cells differentiation from human embryonic stem cells

Mocetinostat (MGCD) which is a kind of histone deacetylase inhibitors (HDACi) promotes human embryonic stem cells (hESCs) differentiation towards neural progenitor cells (NPCs). Application of HDAC inhibitors (HDACi) increased the expression of neuroectodermal markers once neural differentiation was initiated, thereby leading to more NPC generation. We used microarrays to detail the global gene expression during NPC differentiaton upon MGCD treatment and identified the transcript changes effected by MGCD during this process. Undifferentiated H9 hESCs (D0), as well as H9 cells with or without MGCD treatment on day 3 (D3C and D3M) and on day 7(D7C and D7M) of NPC generation were collected for RNA extraction and hybridization on Affymetrix microarrays.

Mocetinostat（MGCD）作为一种组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitors, HDACi），可诱导人类胚胎干细胞（human embryonic stem cells, hESCs）定向分化为神经前体细胞（neural progenitor cells, NPCs）。在神经分化启动阶段，HDACi的处理可上调神经外胚层标记基因的表达水平，进而促进更多NPC的生成。本研究通过基因芯片（microarrays）技术，详细解析了MGCD处理诱导NPC分化过程中的全基因组表达谱，并鉴定了该过程中MGCD介导的转录组变化。我们收集了未分化的H9 hESCs（记为D0组），以及在神经前体细胞生成第3天未接受MGCD处理的D3C组、接受MGCD处理的D3M组，与生成第7天未接受MGCD处理的D7C组、接受MGCD处理的D7M组的H9细胞，用于RNA提取及Affymetrix基因芯片杂交实验。