Xiao et al, Oligodendrocyte Precursor Cells Sculpt the Visual System by Regulating Axonal Remodeling [Dataset]

Raw data from the behavior and imaging experiments of Xiao et al., Nature Neuroscience 2022. For additional details about the acquisition of each part of the dataset, refer to the methods section of the paper. From this dataset, using the published code, all the figures relative to the imaging in the optic tectum can be generated and all the cumulative statistics for the behavioural assays run with Stytra recomputed.

Organisation of the dataset

This dataset is organised in the following subdirectories:
- freely_swimming: contains the data for the freely swimming experiments quantifying motor activity in the various ablated groups. It contains subfolders of groups, each of which contains the Stytra raw data directories for all fish of that group. Refer to `Stytra` and `bouter` documentation for further details about the files.
- OMR: contains the data for the quantification of OMR reflex across different spatial frequencies. It contains subfolders for the control and ablated group, each of which contains the Stytra raw data directories for all fish of that group.
- receptive_field_imaging: contains the imaging data for the receptive field estimation.  Subfolders contains, for each individual fish (both ablated and controls are pooled in the same directory):
    - stytra raw output from the experiment
    - data_from_suite2p_unfiltered.h5: `flammkuchen`-loadable `.h5` file that contains the raw fluorescent trace
    - anatomy.mask: `flammkuchen`-loadable mask file saved by the `pypra` tool that was used for segmenting the tectum, delimiting the region of the tectum
The folder contains an additional file, `manual_alignment_offsets.h5`, where the offsets of the manual morphing across fish were saved.

本数据集源自Xiao等人2022年发表于《自然-神经科学》（Nature Neuroscience）的行为与成像实验原始数据。如需了解数据集各部分的采集细节，请参阅该论文的方法章节。借助已公开的代码，可基于本数据集生成所有与视顶盖（optic tectum）成像相关的图表，并重算所有使用Stytra完成的行为测定的累积统计量。

### 数据集组织形式
本数据集按如下子目录进行组织：
- freely_swimming：包含量化不同消融组运动活动的自由游动实验数据。该目录下包含各组的子文件夹，每个子文件夹均存储对应组中所有实验鱼的Stytra原始数据目录。如需了解文件的更多细节，请参阅`Stytra`与`bouter`的官方文档。
- OMR：包含不同空间频率下视动反射（Optomotor Response, OMR）量化实验的数据。该目录下包含对照组与消融组的子文件夹，每个子文件夹均存储对应组中所有实验鱼的Stytra原始数据目录。
- receptive_field_imaging：包含用于感受野（receptive field）估算的成像数据。该目录下的子文件夹按每条实验鱼划分（消融组与对照组均合并至同一目录），内含以下内容：
- 实验的Stytra原始输出结果
- `data_from_suite2p_unfiltered.h5`：可通过`flammkuchen`加载的.h5格式文件，内含原始荧光轨迹数据
- `anatomy.mask`：可通过`flammkuchen`加载的掩码文件，由用于分割视顶盖、划定其区域的`pypra`工具生成并保存
该文件夹还包含一个额外文件`manual_alignment_offsets.h5`，用于存储跨实验鱼的手动形变偏移量。