Effect of COPI depletion on senescence model systems

To examine effects of COPI depletion on senescence transcriptional signatures we generated cell lines (IMR90 or IMR90 ER:RAS cells) with inducible 2 COPI shRNAs or control shRNAs and induced to senesce with either treatment with Bleomycin or 4OHT treatment. Transcriptional analysis was performed 3 days after induction of doxycycline inducible harpins and 10 days after senescence induction. Comparative gene expression profiling analysis of RNA-seq data for IMR90/IMR90 ER:RAS cells and its KD derivatives (shRNA Control, shCOPB2_1, shCOPB2_2).

为探究包被蛋白复合体I (COPI) 耗竭对细胞衰老转录特征的影响，本研究构建了携带两种诱导型COPI靶向短发夹RNA（shRNA）或对照shRNA的细胞系（IMR90细胞或IMR90 ER:RAS细胞），并通过博来霉素（Bleomycin）处理或4-羟基他莫昔芬（4OHT）处理诱导细胞发生衰老。转录组分析分别于多西环素诱导短发夹RNA表达后3天，以及细胞衰老诱导后10天开展。本研究针对IMR90细胞、IMR90 ER:RAS细胞及其基因敲低（KD）衍生株（对照shRNA组、shCOPB2_1组、shCOPB2_2组）的RNA测序（RNA-seq）数据，完成了比较基因表达谱分析。