In cell LIGR-seq

We performed LIGR-seq in cells infected with the influenza virus. To concentrate viral RNAs in cells, we performed pull-down of viral RNAs by biotinylated antisense ssDNA. 87mut virus has mutations in segment 5 to disrupt the secondary RNA structure of the region. 87rec virus has additional mutations in segment 5 to reconstitute the secondary structure. We set ligation(-) control to negative control samples.

我们在感染流感病毒的细胞中开展了LIGR-seq实验。为富集细胞内的病毒RNA，我们通过生物素标记的反义单链DNA（ssDNA）对病毒RNA进行下拉富集。87mut毒株在第5节段携带突变，以破坏该区域的RNA二级结构；87rec毒株在第5节段携带额外突变，以重建该区域的RNA二级结构。我们将连接阴性对照（ligation(-)）设为阴性对照样本。