Small RNA sequencing facilitates analysis of exosomal RNA transcriptomes

Smal RNA is a type of single-stranded small-molecule RNA with a size of about 18-40 bases, mainly including microRNA, piRNA, snoRNA, snRNA, tRNA and so on. Small RNAs have important regulatory functions in cells and have the potential to be used as disease diagnostic markers or drug targets. We report the results of all small RNAs in exosomes from HTNV infected/uninfected HUVECs by high-throughput sequencing technology. We find that the transcriptomes of Exo-NC group (exosomes from HTNV uninfected cells) and Exo-HV group (exosomes from HTNV infected cells) expressed distinctly different expression patterns of miRNA. Overall design: Examination of exosomes from HTNV infected/uninfected HUVECs

小RNA（small RNA）是一类长度约为18~40个碱基的单链小分子RNA，主要包括微RNA（microRNA）、piRNA、核仁小RNA（snoRNA）、核小RNA（snRNA）、转运RNA（tRNA）等。小RNA在细胞内具有重要的调控功能，具备作为疾病诊断标志物或药物靶点的潜在应用价值。本研究通过高通量测序技术，对汉坦病毒（HTNV）感染与未感染的人脐静脉内皮细胞（HUVECs）外泌体中的全部小RNA进行分析并报道相关研究结果。研究发现，Exo-NC组（未感染HTNV的细胞来源外泌体）与Exo-HV组（感染HTNV的细胞来源外泌体）的microRNA转录组存在显著不同的表达模式。整体实验设计：对汉坦病毒感染/未感染的人脐静脉内皮细胞来源外泌体进行检测。