RNA-sequencing of MDA-MB-231 breast cancer cells with and without treatment of BAY 2506856

MDA-MB-231 cancer cells were treated for 24h with DMSO or BAY 2506856. RNA-sequencing was performed to identify changes induced by treatment on the transcriptome level. Overall design: Cell lines MDA-MB-231 were grown overnight prior to treatment with DMSO or with BAY 2506856 at a concentration of 0.1 µM, 0.3 µM or 1 µM. The cells were harvested 24 h post-treatment for RNA isolation and sequencing.

MDA-MB-231乳腺癌细胞经二甲基亚砜（DMSO）或BAY 2506856处理24小时。本研究通过RNA测序（RNA-sequencing），旨在鉴定该处理在转录组（transcriptome）水平上引发的基因表达变化。实验整体设计：将MDA-MB-231细胞系过夜培养后，分别采用DMSO，或浓度为0.1 μM、0.3 μM、1 μM的BAY 2506856进行处理；于处理后24小时收集细胞，用于RNA提取与测序。