Stenotrophomonas maltophilia FLR19 response to pH

We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes. Bacterial transcriptional response to acidic, neutral and basic pH

本研究旨在明确一株源自囊性纤维化（cystic fibrosis）患者的嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）分离株对相关pH梯度（pH 5、7和9）的响应模式，实验通过将该菌株培养于磷酸盐缓冲培养基中，并开展RNAseq实验完成。本研究数据表明，酸性环境对FLR19菌株具有胁迫作用，该菌株可通过上调应激响应基因与抗生素抗性基因的表达来产生应对应答。细菌在酸性、中性及碱性pH条件下的转录响应