C. elegans miRNAs conserved in D. melanogaster and H. sapiens.

73 C. elegans miRNAs have significant identity at their 5�� ends and/or ��70% similarity over their entire sequences to both fly and human miRNAs. All the 73 C. elegans miRNAs have 5�� related sequences in both flies and humans, whereas 16/73 C. elegans miRNAs are also classified as ��70% homologous over length to miRNAs in flies and humans. For detail in sequence relationships refer to Figure S1. ?indicates miRNAs with 5�� end sequence homology present in worms, flies and humans. Superscript 70 denotes miRNAs with ��70% similarity over full sequence across the three analyzed species.It should be noted that the miRNA registry was extensively modified in the year 2007 (releases 10.0 and 10.1), introducing changes to previous mature miRNA sequences as well as adding new mature miRNA sequences to C. elegans (5), D. melanogaster (75) and human (494) miRNA databases. We performed our analysis using the latest miRBase release (10.1). We elected to use C. elegans sequences as reference anchors because of the general availability of deletions for mir genes.

73种秀丽隐杆线虫（Caenorhabditis elegans, C. elegans）的微小RNA（microRNA, miRNA）在其5'端具有显著同源性，和/或在全长序列上与果蝇和人类的miRNA存在≥70%的序列相似性。所有73种秀丽隐杆线虫miRNA在果蝇和人类中均存在与其5'端序列相关的同源序列；其中16/73的秀丽隐杆线虫miRNA同时满足：在全长序列上与果蝇和人类的miRNA具有≥70%的同源性。欲了解序列关联的详细信息，请参见补充图S1。上标？代表在秀丽隐杆线虫、果蝇和人类中均存在5'端序列同源性的miRNA；上标70则代表在三种分析物种的全长序列上具有≥70%相似性的miRNA。
需说明的是，miRNA登记库（miRBase）于2007年进行了大规模修订（版本10.0与10.1），不仅对既往成熟miRNA序列进行了调整，还为秀丽隐杆线虫、黑腹果蝇（Drosophila melanogaster, D. melanogaster）和人类的miRNA数据库分别新增了5、75和494条成熟miRNA序列。本研究采用最新版miRBase 10.1开展分析工作。鉴于mir基因缺失突变体的通用性较强，本研究选择以秀丽隐杆线虫的miRNA序列作为参考锚点。