Obesity-related T cell dysfunction impairs immunosurveilance and increases cancer risk

The current study used a mouse model of diet-induced obesity to investigate tumor-infiltrating CD8+ T cell responses. Tumors from lean or obese C57Bl/6J mice were harvested and CD8 T cells were isolated by CD8 (TIL) mouse magnetic Microbeads (Miltenyi) and Flurescence-activated cell sorting.

本研究采用饮食诱导肥胖（diet-induced obesity）小鼠模型，探究肿瘤浸润性CD8+ T细胞（tumor-infiltrating CD8+ T cell）的应答特性。研究人员采集瘦弱及肥胖C57Bl/6J小鼠的肿瘤组织，通过CD8阳性肿瘤浸润淋巴细胞（tumor-infiltrating lymphocytes，TIL）小鼠磁性微珠（美天旎，Miltenyi）及荧光激活细胞分选（fluorescence-activated cell sorting）分离得到CD8 T细胞。