Arabidopsis thaliana Raw sequence reads

a mutant allele of HDA6, shi5, was identified through EMS mutagenesis and map-based cloning. The mutant shi5 shows some phenotypes not reported previously. Using total RNA samples extracted from 10d-old seedlings growing on 1/2MS agar plates were used for the RNA sequencing. The wild type is a transgenic line containing PSOT12-LUC contruct in the Col background. This line is used as the materials for mutagenesis to produce and screen shi5.

通过乙基甲磺酸酯（EMS）诱变与图位克隆技术，研究者鉴定得到组蛋白去乙酰化酶6（HDA6）的突变等位基因shi5。该shi5突变体呈现出此前未被报道的表型。以在1/2MS琼脂平板上培养的10日龄幼苗提取的总RNA为材料，开展RNA测序实验。野生型为在哥伦比亚（Col）生态型背景下整合PSOT12-LUC构建体的转基因株系，该株系被用作诱变材料以创制并筛选shi5突变体。