Yeast (S. cerevisiae SK1) Sporulation/Meiosis

Transription profile of Saccharomyces cerevisiae SK1 cultures undergoing synchronous sporulation. We have measured mRNA levels in synchronized SK1 cells immediately upon transfer to the sporulation medium and every 30 minutes after that for 6 hours. mRNA extracted from these cultures were converted to cDNA and hybridized to microarrays and log2 ratios of hybridization signal of each time point was compared to that of time zero (immediately prior to transfer to the sporulation medium). Keywords: Time course RNA from time zero is control, other time samples are experimental, two replicates per array, dye reverse of each time point on separate arrays, each time sample represents pooled cells from six independent synchronized cultures cultivated on the same day.

本数据集涵盖同步孢子形成过程中酿酒酵母（Saccharomyces cerevisiae）SK1菌株培养物的转录组谱信息。研究人员检测了同步化SK1细胞在转移至孢子培养基瞬间及后续6小时内每30分钟的mRNA水平。从上述培养物中提取的mRNA经反转录为互补脱氧核糖核酸（cDNA）后，与微阵列（microarray）进行杂交；将每个时间点的杂交信号log₂比值与零时间点（即转移至孢子培养基前瞬间的对照样本）的信号比值进行比较。关键词：时间进程实验；零时间点的RNA作为对照，其余时间点样本为实验组；每张微阵列设置2次重复；每个时间点样本在独立微阵列上开展染料反转实验；每个时间点样本均为当日培养的6份独立同步化培养物的混合细胞。