Circulating miRNAs Bear a Signature of Acute Hepatitis C Viremia. Homo sapiens

miRNAs were purified from frozen plasma from injection drug users in the Baltimore Before and After the Start of Hepatitis (BBAASH) Study before, during and after acute hepatitis C infection. Acute HCV outcome of 'Clear' indicates spontaneous resolution of infection. 'Time 3' in these individuals indicates the last known viremic specimen from these individuals before resolution and 'Time 4' indicates a plasma sample greater than one month after resolution of infection. Acute HCV outcome of 'Persist' indicates progression to chronic infection and 'Time 3' and 'Time 4' samples from these individuals are plasma samples time-matched to those of the clearers with the same name. Overall design: qPCR miRNA expression profiling. 22 individuals HCV negative at enrollment were profiled for miRNA abundance in plasma, before, during, and after HCV infection.

研究人员从巴尔的摩肝炎发生前后研究（BBAASH）中注射毒品使用者的冻存血浆中提取了微小核糖核酸（miRNAs），样本采集时间覆盖急性丙型肝炎病毒（HCV）感染前、感染期间及感染后。急性HCV感染的「清除」结局表示感染自发清除；该队列中「时间点3」指感染清除前最后一份可检测到病毒血症的血浆样本，「时间点4」指感染清除后超过1个月采集的血浆样本。急性HCV感染的「持续」结局表示感染进展为慢性感染，该队列中「时间点3」与「时间点4」样本为与同名自发清除者时间匹配的血浆样本。实验设计：采用定量聚合酶链式反应（qPCR）开展miRNA表达谱分析。本研究对22名入组时HCV检测呈阴性的个体，在HCV感染前、感染期间及感染后三个阶段的血浆中miRNA表达丰度进行了检测分析。