Gene expression profiling of IOERT exposed MCF10A breast epithelial cells. Homo sapiens

Intraoperative Electron Radiation Therapy (IOERT) is a therapeutic technique that delivers a single high dose of ionizing radiation (IR) directly to the tumor bed during cancer surgery. The main goal of IOERT is to counteract tumor growth by acting on residual cancer cells in the site at high risk for recurrence as well as to preserve healthy surrounding tissue from the side effects of radiation therapy. The high IR dose used during IOERT treatment induces a strong stress response resulting in the activation of pro- and anti-proliferative cell signaling pathways in both tumor and normal cells. The radiobiology of healthy tissue response to IR is a topic of interest which may contribute to avoid the impairment of normal tissue/organ function and to decrease the risks of secondary cancers. In this study, we examined changes in gene expression in MCF10A breast epithelial cell line exposed to 9Gy and 23Gy high single dose of IR delivered by IOERT. Overall design: Changes in gene expression in MCF10A breast epithelial cell line exposed to 9Gy and 23Gy high single dose of IR (named MCF10A_9Gy and MCF10A_23Gy respectively), were analyzed as two-color hybridizations using Agilent Technologies whole human genome 4x44K microarrays

术中电子放射治疗（Intraoperative Electron Radiation Therapy, IOERT）是一种在癌症手术过程中，将单次高剂量电离辐射（ionizing radiation, IR）直接施加于肿瘤床的治疗技术。该技术的主要目标为作用于复发高风险位点处的残留癌细胞以抑制肿瘤生长，同时保护周围健康组织免受放射治疗的副作用影响。IOERT治疗中使用的高剂量电离辐射可诱导强烈的应激反应，进而激活肿瘤细胞与正常细胞内的促增殖与抗增殖细胞信号通路。健康组织对电离辐射的放射生物学反应是当前的研究热点，该领域的研究有助于规避正常组织/器官的功能损伤，并降低继发癌症的发生风险。本研究针对经IOERT给予9Gy与23Gy单次高剂量电离辐射的MCF10A乳腺上皮细胞系，分析其基因表达变化。整体实验设计：采用安捷伦科技（Agilent Technologies）全人类基因组4x44K微阵列开展双色杂交实验，对经9Gy与23Gy单次高剂量电离辐射处理的MCF10A乳腺上皮细胞系（分别命名为MCF10A_9Gy与MCF10A_23Gy）的基因表达变化进行检测分析。