High-light stress RNA-seq Marchantia polymorpha

In order to elucidate the role of the single Marchantia B-GATA ortholog in response to high light intensities, a transcriptomic analysis of Marchantia polymorpha BoGa, Mpb-gata1 mutants and MpB-GATA1ox under high-ligh stress conditions was performed. Transcriptome comparison including three replicate measurements of three biological samples. Plants were subjected to three different conditions: six hours of high-light (HL) with 1200 µmol m-2 s-1 at 20-22°C (t6HL), six hours of mock treatment with 60-70 µmol m-2 s-1 at 20-22°C (t6G­L), or at time point 0h (t0).

为阐明地钱（Marchantia polymorpha）单拷贝B-GATA同源基因（B-GATA ortholog）在高光胁迫响应中的作用，本研究对高光胁迫条件下的地钱BoGa、Mpb-gata1突变体以及MpB-GATA1过表达株系开展了转录组分析。该转录组比较分析涵盖三份生物学样本的三次重复检测。实验设置了三种处理条件：在20-22℃环境下，以1200 μmol·m⁻²·s⁻¹光照强度处理6小时的高光组（t6HL）、以60-70 μmol·m⁻²·s⁻¹光照强度处理6小时的模拟处理组（t6GL），以及0小时时间点对照组（t0）。