A novel paracrine induction of RNA granules by feline calicivirus. A novel paracrine induction of RNA granules by feline calicivirus

In this study we are examining the paracrine effect induced by feline calicivirus (FCV) infection on stress granule (SG) accumulation. We provided an understanding of paracrine granules function and specificity through their affinity purification followed RNAseq to systematically analyse their RNA content. Overall design: We purified in triplicate a granule enriched fraction from GFP-G3BP1 U2OS cells treated (and not) with the virus free supernatant. The RNA was then purified, sequenced and compared to the total RNA.

本研究旨在探究猫杯状病毒（feline calicivirus, FCV）感染诱导的旁分泌效应对应激颗粒（stress granule, SG）聚集的影响。本研究通过对旁分泌颗粒进行亲和纯化并结合RNA测序，系统分析其RNA组成，以此阐明该类颗粒的功能与作用特异性。
总体实验设计：我们以三次生物学重复，从经无病毒上清液处理以及未处理的表达GFP-G3BP1的U2OS细胞中纯化得到颗粒富集组分。随后对其中的RNA进行提取、纯化、测序，并与总RNA样本进行对比分析。