Metagenome assembled genomes co_5-Bin_46_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

本研究中的宏基因组共组装序列使用CONCOCT工具在anvi’o v5.1平台中完成宏基因组分箱：将长度不小于2500 bp的支架序列聚类为宏基因组组装基因组（MAGs），并在anvi’o平台内对其进行人工优化校正。此外，使用dRep v2.3.2工具对所得MAGs进行去冗余处理，通过设置参数标记‘-comp 50 -con 10’并采用默认参数，获取代表性基因组。