Table_4_Comparative transcriptomic and proteomic analysis reveals common molecular factors responsive to heat and drought stresses in sweetpotaoto (Ipomoea batatas).xlsx

IntroductionCrops are affected by various abiotic stresses, among which heat (HT) and drought (DR) stresses are the most common in summer. Many studies have been conducted on HT and DR, but relatively little is known about how drought and heat combination (DH) affects plants at molecular level.MethodsHere, we investigated the responses of sweetpotato to HT, DR, and DH stresses by RNA-seq and data-independent acquisition (DIA) technologies, using controlled experiments and the quantification of both gene and protein levels in paired samples.ResultsTwelve cDNA libraries were created under HT, DR, and DH conditions and controls. We identified 536, 389, and 907 DEGs in response to HT, DR, and DH stresses, respectively. Of these, 147 genes were common and 447 were specifically associated with DH stress. Proteomic analysis identified 1609, 1168, and 1535 DEPs under HT, DR, and DH treatments, respectively, compared with the control, of which 656 were common and 358 were exclusive to DH stress. Further analysis revealed the DEGs/DEPs were associated with heat shock proteins, carbon metabolism, phenylalanine metabolism, starch and cellulose metabolism, and plant defense, amongst others. Correlation analysis identified 6465, 6607, and 6435 co-expressed genes and proteins under HT, DR, and DH stresses respectively. In addition, a combined analysis of the transcriptomic and proteomic data identified 59, 35, and 86 significantly co-expressed DEGs and DEPs under HT, DR, and DH stresses, respectively. Especially, top 5 up-regulated co-expressed DEGs and DEPs (At5g58770, C24B11.05, Os04g0679100, BACOVA_02659 and HSP70-5) and down-regulated co-expressed DEGs and DEPs (AN3, PMT2, TUBB5, FL and CYP98A3) were identified under DH stress.DiscussionThis is the first study of differential genes and proteins in sweetpotato under DH stress, and it is hoped that the findings will assist in clarifying the molecular mechanisms involved in sweetpotato resistance to heat and drought stress.

农作物受多种非生物胁迫的影响，其中热胁迫（HT）和干旱胁迫（DR）在夏季尤为常见。针对HT和DR的研究已颇丰，然而关于干旱与热胁迫组合（DH）对植物分子层面影响的研究相对较少。本研究通过RNA测序和独立数据采集（DIA）技术，采用控制实验方法，对甘薯在HT、DR和DH胁迫条件下的基因和蛋白质水平进行量化，从而探究了甘薯对这些胁迫的响应。结果表明，在HT、DR和DH胁迫条件下，分别鉴定出536、389和907个差异表达基因（DEGs）。其中，147个基因在三种胁迫条件下均有所表达，而447个基因则与DH胁迫特异性相关。蛋白质组学分析发现，与对照相比，在HT、DR和DH处理下分别鉴定出1609、1168和1535个差异表达蛋白（DEPs），其中656个为共同表达，358个为DH胁迫特异性表达。进一步分析显示，DEGs/DEPs与热休克蛋白、碳代谢、苯丙氨酸代谢、淀粉和纤维素代谢以及植物防御等相关。相关性分析揭示，在HT、DR和DH胁迫条件下，分别鉴定出6465、6607和6435个共表达基因和蛋白。此外，转录组和蛋白质组数据的联合分析显示，在HT、DR和DH胁迫条件下，分别鉴定出59、35和86个显著共表达DEGs和DEPs。特别是，在DH胁迫条件下，还鉴定出前5个上调共表达DEGs和DEPs（At5g58770、C24B11.05、Os04g0679100、BACOVA_02659和HSP70-5）以及下调共表达DEGs和DEPs（AN3、PMT2、TUBB5、FL和CYP98A3）。讨论部分指出，这是首次对甘薯在DH胁迫条件下差异表达基因和蛋白的研究，期望研究结果能有助于阐明甘薯抵抗热胁迫和干旱胁迫的分子机制。