Assembled mRNAs from potato genotypes contrasted for their hexanal isomerase activity

From PacBio and Illumina mRNA-seq coming from leaf total RNA isolated from three <em>Solanum tuberosum</em> genotypes (Desiree, Phureja153 and S869), mRNA isoforms were assembled. <strong>Phureja153:</strong> low (3Z):(2E) hexenal isomerase activity <strong>Desiree:</strong> mid (3Z):(2E) hexenal isomerase activity <strong>S869</strong>: high (3Z):(2E) hexenal isomerase activity <strong>== Step1: PacBio subreads to FASTA and GTF annotation == </strong> For each genotype, a FASTA file and a GTF annotation file are available. File is called "pacbio_assembled_isoforms.tar.gz". Pipeline used: https://github.com/SilkeAllmannLab/pacbio_snakemake/releases/tag/v0.1.0 Raw PacBio data submitted to NCBI EBI ENA under accession PRJXXX. === Step 2: SQANTI QC ==== SQANTI3 was run on these three PacBio assembled sets of transcript to correct them. The corrected transcripts and related SQANTI3 info are available in the "sqanti_corrected_transcripts.tar.gz" file.从提取自三个马铃薯（Solanum tuberosum）基因型（Desiree、Phureja153与S869）叶片总RNA的PacBio及因美纳（Illumina）mRNA测序（mRNA-seq）数据中，完成了mRNA转录本异构体的组装。**Phureja153：** 具有低水平(3Z):(2E)己烯醛异构酶活性 **Desiree：** 具有中等水平(3Z):(2E)己烯醛异构酶活性 **S869：** 具有高水平(3Z):(2E)己烯醛异构酶活性 **== 步骤1：PacBio子读段转换为FASTA格式及GTF注释 ==** 针对每个基因型，均提供了FASTA格式文件与GTF注释文件，所有文件均打包至"pacbio_assembled_isoforms.tar.gz"压缩包中。本次分析使用的流程为：https://github.com/SilkeAllmannLab/pacbio_snakemake/releases/tag/v0.1.0。原始PacBio测序数据已提交至美国国家生物技术信息中心（NCBI）、欧洲生物信息研究所（EBI）与欧洲核苷酸档案馆（ENA）数据库，登录号为PRJXXX。 **=== 步骤2：SQANTI质量控制（QC） ===** 针对这三套PacBio组装得到的转录本集，运行SQANTI3进行校正。校正后的转录本及相关SQANTI3分析信息均包含在"sqanti_corrected_transcripts.tar.gz"文件中。