来自四种不同颜色大豆品种的代谢组学数据

50 ± 1 mg冻干样品曾赎金红2 mL EP管中，加入1000 μL预冷提取混合物（甲醇：水，v/v = 3：1）和10 μL核糖醇是加编辑。离心30秒后，加入陶瓷珠，将试管置于35Hz的球磨机中4分钟。然后将试管浸入冰水中并超声处理 5 分钟。在 4 °C 下以 10,000 rpm 离心 15 分钟后，将 200 μL 上清液转移到新的 1.5 mL EP 管中并在真空浓缩器中浓缩。干燥后，加入120μL盐酸甲氧胺（20mg/mL吡啶）。T溶液是 g均匀编辑孵育d30分钟。接下来，向每个样品中加入 120 μL BSTFA 试剂（1% TMC，v/v），然后在 70 °C 下孵育 1.5 小时。 冷却至室温后，加入7 μL FAMEs，气相色谱法分析。

使用Agilent 7890气相色谱仪（GC）和配备DB-5ms毛细管柱的飞行时间质谱仪对代谢物进行分析。将1μL等分试样的样品提取物在230°C下注入进样口，流速为1mL/min。初始温度设置为50 °C，持续1 min，然后以10 °C/min的速度升至310 °C，并在310 °C下保持8 min。在全扫描模式下以每秒12.5个光谱的速率采集光谱数据，质量范围为50-500 m/z。

50 ± 1 mg lyophilized sample was placed into a 2 mL EP tube, followed by the addition of 1000 μL pre-cooled extraction mixture (methanol:water, v/v = 3:1) and 10 μL ribitol. After centrifugation for 30 seconds, ceramic beads were added, and the tube was subjected to homogenization in a ball mill at 35 Hz for 4 minutes. The tube was then immersed in ice water and sonicated for 5 minutes. Following centrifugation at 10,000 rpm for 15 minutes at 4 °C, 200 μL of the supernatant was transferred to a new 1.5 mL EP tube and concentrated in a vacuum concentrator. After drying, 120 μL of methoxyamine hydrochloride (20 mg/mL in pyridine) was added, and the mixture was incubated uniformly for 30 minutes. Next, 120 μL of BSTFA reagent (1% TMC, v/v) was added to each sample, followed by incubation at 70 °C for 1.5 hours. After cooling to room temperature, 7 μL of FAMEs was added, and the sample was analyzed by gas chromatography.

Metabolites were analyzed using an Agilent 7890 gas chromatography (GC) system coupled with a time-of-flight mass spectrometer equipped with a DB-5ms capillary column. A 1 μL aliquot of the sample extract was injected into the GC inlet at 230 °C, with a carrier gas flow rate of 1 mL/min. The initial oven temperature was set at 50 °C for 1 minute, then ramped to 310 °C at a rate of 10 °C/min, and held at 310 °C for 8 minutes. Spectral data were acquired in full-scan mode at a rate of 12.5 spectra per second, with a mass range of 50–500 m/z.