Raw data of "Culture Breakthrough Enables Goose Circovirus (GoCV) Isolation and Pathogenic Gosling Modeling to Uncover the Association between Viral Infection and Vaccine Failure."

Goose circovirus (GoCV), recognized as the most economically significant immunosuppressive pathogen in global waterfowl production, has caused considerableeconomic losses annually, andmultiprovincial surveillance studies have documentedhigh seroprevalence rates. The critical barrier to combating GoCV has persisted in the complete absence of viable in vitroculture systems, fundamentally limitingpathogenesis studies and vaccine development. In a breakthrough advancement, this study pioneers the first successful establishment of a stable primary goose embryo kidney (GEK) cell culture system for GoCV propagation, achieving sustained viral replication through innovative culture protocol optimization. Comprehensive virological characterization via synchronized growth curve analysis, transmission electron microscopy, and IFA quantification not only confirms efficient viral proliferation but also resolves longstanding debates regarding theintracellular replication mechanisms of GoCV,termed GoCV/369/2020. Pathogenesistrials involvinggosling with the novelisolated GoCV revealed unprecedented clinicopathologicalcorrelations, revealingsevere growth retardation, feather disorder, persistent fecal shedding, and biphasic organ damage progression from acute hypertrophy to chronic atrophy. Most critically, we provide the first experimental evidence linking GoCV infection to catastrophic vaccine failure, showing reductions in antibody titers of avian influenza virus and Newcastle disease virus . This breakthrough has led to the establishment ofpoultry industry solutions, such asstandardized viral isolation for diagnostics, optimized vaccination schedules, and targeted biosecurity against fecal-oral transmission. The integrated strategy reduces disease incidence and improves productivity in GoCV-affected areas, addressing both urgent control needs and sustainable farming goals. By connecting virology with practical applications, it advances research methods and enhances global waterfowl production security.

鹅圆环病毒（Goose circovirus）是全球水禽养殖业中经济影响最为显著的免疫抑制性病原体，每年均造成巨额经济损失，多省份监测研究均已证实其存在高血清阳性率。当前防控鹅圆环病毒的核心瓶颈始终在于完全缺乏可行的体外培养体系，从根本上制约了其发病机制研究与疫苗研发。
本研究取得突破性进展，首次成功建立了用于鹅圆环病毒增殖的稳定原代鹅胚肾（Goose Embryo Kidney, GEK）细胞培养体系，并通过创新性优化培养方案实现了病毒的持续复制。通过同步化生长曲线分析、透射电子显微镜及免疫荧光定量（Immunofluorescence Assay, IFA）开展的全面病毒学特性分析，不仅证实了病毒的高效增殖，还澄清了鹅圆环病毒细胞内复制机制这一长期存在的学术争议，本次研究所用分离毒株命名为GoCV/369/2020。
针对该新分离毒株的雏鹅致病性试验揭示了前所未有的临床病理关联特征，表现为严重生长迟缓、羽毛发育异常、持续粪便排毒，以及从急性肥大向慢性萎缩转变的双相性器官损伤进程。尤为关键的是，本研究首次提供实验证据，证实鹅圆环病毒感染可引发灾难性的疫苗免疫失败，结果显示禽流感病毒与新城疫病毒的抗体滴度显著下降。
该突破成果已推动形成适配禽养殖业的综合解决方案，包括用于临床诊断的标准化病毒分离技术、优化的免疫程序，以及针对粪口传播路径的针对性生物安全防控措施。该综合防控策略可降低鹅圆环病毒疫区的疾病发病率，提升生产性能，同时兼顾紧急防控需求与可持续养殖目标。本研究将病毒学基础研究与产业应用相结合，推动了相关研究方法革新，同时提升了全球水禽养殖业的安全保障水平。