CIL:28779

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.

本数据集为OD31品系中，经RNA干扰（RNA interference, RNAi）耗竭秀丽隐杆线虫着丝粒蛋白A（CeCENP-A）且表达GFP-KNL-2的秀丽隐杆线虫胚胎的第一次有丝分裂过程的转盘共聚焦延时成像结果。前期阶段可观测到弥散分布的细胞核GFP信号。然而与对照组（CIL 28778）不同，该样本直至有丝分裂后期起始前均未观测到清晰的GFP焦点；直至后期起始时刻，GFP-KNL-2焦点沿纺锤体及染色质表面形成。该成像视频时长约18分钟，成像视野上下方向跨度约30微米，图像采集间隔为10秒。