Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and SNORD12B Knock Down Expression of ESCC Transcriptomes.. Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and SNORD12B Knock Down Expression of ESCC Transcriptomes.

We aimed to detect the mRNA expression levels in KYSE30 cells after stable knock down SNORD12B expression with shRNA or vector. Overall design: Totally, three samples were examined: KYSE30 stable knock down SNORD12B cell lines (sh12B-#1 and sh12B#2) versus pLKO.1.本研究旨在检测KYSE30细胞经短发夹RNA（shRNA）介导的SNORD12B表达稳定敲低，或空载载体处理后的信使核糖核酸（mRNA）表达水平。实验整体设计：共计检测3组样本：KYSE30稳定敲低SNORD12B的细胞系（sh12B-#1与sh12B#2）与pLKO.1载体对照组。