Impacts of red tide on microbial network complexity and stability of Noctiluca scintillans in the Qinhuangdao coast

Red tide is common in the Bohai Sea off the coast of Qinhuangdao, and one of the primary algae species that causes it there is Noctiluca scintillans. Abiotic and biotic variables work together to determine the occurrence, development, and extinction of red tides. Microbial interactions have been shown to be one of the most important biotic factors in the occurrence and development of red tide. However, it is still unclear how these interactions react to environmental changes and affect the red tide process during N. scintillans bloom outbreaks. Using high-throughput sequencing of 16S rRNA and 18S rRNA genes and microbial co-occurrence network analysis, we examined the microbial community structure, microbial interactions, the composition of key species, and the relationship between microbes and environmental factors under the influence of N. scintillans blooms in the offshore region of Qinhuangdao. Our goal was to uncover the role of microbial interactions and other biotic factors in the occurrence of the N. scintillans blooms. Finding out how microbial interactions and other biological elements contribute to N. scintillans blooms is the goal.

秦皇岛近岸渤海海域赤潮频发，该海域赤潮的主要引发藻种之一为夜光藻（Noctiluca scintillans）。非生物与生物因子协同调控赤潮的发生、发展与消亡全过程。微生物互作已被证实为影响赤潮发生与发展的核心生物因子之一。然而，在夜光藻赤潮暴发阶段，此类微生物互作如何响应环境变化并调控赤潮进程，目前仍未明晰。本研究通过对16S rRNA与18S rRNA基因进行高通量测序（high-throughput sequencing），并结合微生物共现网络分析（microbial co-occurrence network analysis），针对秦皇岛近岸海域在夜光藻赤潮影响下的微生物群落结构、微生物互作模式、关键物种组成，以及微生物与环境因子间的关联关系开展了系统探究。本研究旨在揭示微生物互作与其他生物因子在夜光藻赤潮发生过程中的作用机制。明确微生物互作与其他生物因子如何促成夜光藻赤潮的发生，亦是本研究的核心目标。