Transcriptomic computational analysis of mastic oil-treated Lewis lung carcinomas

Mastic oil from Pistacia lentiscus variation chia, a blend of bioactive terpenes with recognized medicinal properties, has been recently shown to exert anti-tumor activity. Lewis lung carcinoma (LLC) cells are mastic oil-susceptible cells and were used in this work to study the effects of mastic oil at the transcriptomic level. Expression profiling by array. LLC cells were treated with mastic oil or DMSO (control) for 3h, 6h, 12h, 24h, or 48h. Two replicates per treatment/time point. Data were normalized using rank invariant and quantile normalization.

来自乳香黄连木（Pistacia lentiscus）chia变种的乳香精油，是一类兼具公认药用活性的生物活性萜类混合物，近期研究证实其具有抗肿瘤活性。刘易斯肺癌（Lewis lung carcinoma, LLC）细胞为对乳香精油敏感的细胞系，本研究采用该细胞系在转录组层面探究乳香精油的生物学效应，实验通过芯片技术开展表达谱分析：将LLC细胞分别用乳香精油或二甲基亚砜（DMSO，对照组）处理3小时、6小时、12小时、24小时或48小时，每个处理/时间点设置2次生物学重复；数据采用秩不变（rank invariant）与分位数归一化（quantile normalization）方法进行标准化处理。