Profiling 11,639 genes in PBMCs from 2 donors: U133 A 2.0 Custom CDF Version 9

The immune responses generated by YF-17D by profiling 11,639 genes in PBMCs from 2 donors cultured with YF-17D vaccine were accessed after 3 and 12 hours. Keywords: time course, in vitro stimulaion PMBCs were isolated with citrate buffered cell preparation tubes (CPT) from 2 healthy donors who were not previously vaccinated with YF-17D. Total RNA was either isolated directly from the freshly collected cells or after cell culture. Cells were cultured for 3 and 12 hours in the presence or absence of YF-17D vaccine.

本数据集通过对2名既往未接种YF-17D疫苗的健康供者的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）开展11639个基因的表达谱分析，以评估YF-17D疫苗诱导的免疫应答；上述经YF-17D疫苗培养的细胞，分别于培养3小时及12小时后完成检测。
关键词：时间进程、体外刺激。
外周血单个核细胞（PBMCs）采用枸橼酸盐缓冲液细胞制备管（Citrate Buffered Cell Preparation Tubes, CPT）分离获取。总RNA可直接从新鲜采集的细胞中提取，亦可于细胞培养结束后提取。细胞分别在添加YF-17D疫苗与未添加YF-17D疫苗的条件下培养3小时及12小时。