Effects of grape powder supplementation on inflammatory and antioxidant markers in hemodialysis patients: A randomized double-blind study

Introduction: Polyphenols contained in natural sources such as grapes, have been considered pharmacological agents to combat oxidative stress and inflammation, common features in Chronic Kidney Disease patients. Objective: To evaluate the effects of grape powder supplementation on inflammatory and antioxidant biomarkers in hemodialysis (HD) patients. Methods: The double-blind placebo-controlled randomized clinical trial evaluated non-diabetic HD patients that received grape powder (500 mg of polyphenols/day) (n = 16, 9 men, 53.0 ± 9.8 years of age, 111.6 ± 58.2 HD months) or placebo (n = 16, 9 men, 52.7 ± 13.7 years of age, 110.4 ± 93.1 HD months) for five weeks. The glutathione peroxidase (GSH-Px) activity and C-reactive protein (CRP) levels were evaluated by ELISA method. Results: After the intervention period, the patients receiving grape powder showed an increase in the GSH-Px activity (16.5 (41.0) to 42.0 (43.3) nmol/min/ml) (p

引言：葡萄等天然来源中含有的多酚类物质，已被视为可对抗氧化应激与炎症反应的药理活性制剂，而氧化应激和炎症正是慢性肾脏病（Chronic Kidney Disease）患者的常见病理特征。研究目的：评估葡萄粉补充干预对血液透析（hemodialysis, HD）患者炎症与抗氧化生物标志物的影响。研究方法：本项双盲安慰剂对照随机临床试验纳入非糖尿病血液透析患者，将其随机分为两组：葡萄粉干预组（每日摄入含500mg多酚的葡萄粉，n=16，其中男性9例，年龄53.0±9.8岁，血液透析时长111.6±58.2个月）与安慰剂组（n=16，其中男性9例，年龄52.7±13.7岁，血液透析时长110.4±93.1个月），干预周期为5周。本研究采用酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）检测谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-Px）活性与C反应蛋白（C-reactive protein, CRP）水平。研究结果：干预周期结束后，葡萄粉干预组患者的谷胱甘肽过氧化物酶活性从16.5(41.0)提升至42.0(43.3) nmol/min/ml，(p