Ribosome profiling under ABCE1 depletion to study aberrant translation termination

We report the effect of siRNA-mediated ABCE1 KD in HeLa cells, using a modified ribosome profiling protocol adapted from Ingolia et al., 2011 in order to specifically investigate ABCE1 role on translation termination Overall design: Control and ABCE1 KD conditions, three replicates for condition. totalRNA samples in parallel for input control.

本研究报道了小干扰RNA（siRNA）介导的ABCE1基因敲低（KD）在海拉（HeLa）细胞中的作用效果。本研究采用改编自Ingolia等2011年研究的改良型核糖体谱分析（ribosome profiling）实验方案，以专门探究ABCE1在翻译终止过程中的功能。实验设计概述：设置对照组与ABCE1基因敲低组，每组设置3个生物学重复；同时平行采集总RNA（totalRNA）样本作为输入对照。