Genome-wide transcriptome analysis of VC and VHL-KO Caki-1 cells.

To compare the transcriptomic changes mediated by VHL knockout in Caki-1 cells, we carried out the next-generation sequencing (NGS)-derived transcriptomic profiling. We found significant enrichment of gene signatures involving IFNa/b signaling in VHL-KO Caki-1 cells compared to control cells. Our analysis also demonstrated that VHL deficiency induced the expression of genes associated with the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway involved in sensing the cytoplasmic presence of double-stranded DNA (dsDNA). mRNA expression profiles of control and VHL-KO Caki-1 cells.

为比较Caki-1细胞中VHL基因敲除所介导的转录组变化，我们开展了基于下一代测序（next-generation sequencing, NGS）的转录组谱分析。相较于对照组细胞，VHL敲除的Caki-1细胞中显著富集了涉及干扰素α/β（IFNα/β）信号通路的基因特征。本分析还证实，VHL缺陷可诱导与环鸟苷酸-腺苷酸合成酶（cyclic GMP-AMP synthase, cGAS）-干扰素基因刺激因子（stimulator of interferon genes, STING）通路相关的基因表达，该通路参与感知细胞质内双链DNA（double-stranded DNA, dsDNA）的存在。本数据集涵盖对照组与VHL敲除Caki-1细胞的mRNA表达谱。