A transcriptomics approach identifies DR5-AS as a cisplatin-inducible long non-coding RNA that modulates cell cycle and proliferation. A transcriptomics approach identifies DR5-AS as a cisplatin-inducible long non-coding RNA that modulates cell cycle and proliferation

We have performed a transcriptomics study in which we first applied 80 uM of cisplatin for 16h. Total RNA was isolated from control as well as drug treated cells and apoptosis induction was confirmed by Annexin V and 7AAD staining. Total RNAs were subjected to deep-sequencing to identify differentially expressed RNAs. We then took advantage of bioinformatic tools to identify which lncRNAs are differentially expressed upon cisplatin treatment Overall design: Total RNA was isolated from negative control (0.1 % DMSO) and cisplatin (80 uM, 16h) treated HeLa cells in triplicate to perform deep-sequencing. 0.1% DMSO was used as control and denoted as 150605_SND405_A_L007_GDU-14, 150605_SND405_A_L007_GDU-15, 150605_SND405_A_L007_GDU-16.

本研究开展了一项转录组学分析：首先使用80 μM顺铂（cisplatin）处理细胞16小时。分别从阴性对照组与药物处理组细胞中提取总RNA，并通过膜联蛋白V（Annexin V）与7-氨基放线菌素D（7AAD）染色验证了细胞凋亡的诱导情况。随后对总RNA进行深度测序以筛选差异表达RNA，再借助生物信息学工具，鉴定出顺铂处理后发生差异表达的长链非编码RNA（lncRNAs）。
实验设计：以0.1%二甲基亚砜（DMSO）作为阴性对照，采用80 μM顺铂处理海拉（HeLa）细胞16小时，每组设置3次生物学重复，提取总RNA后开展深度测序。其中0.1% DMSO对照组的样本编号分别为150605_SND405_A_L007_GDU-14、150605_SND405_A_L007_GDU-15及150605_SND405_A_L007_GDU-16。