Additional file 1: of Methylated genomic loci encoding microRNA as a biomarker panel in tissue and saliva for head and neck squamous cell carcinoma

Figure S1. Flow chart of mgmiRs search for HNSCC. Figure S2. Screening of mgmiR in HNSCC using HNSCC and control cell lines. Relative methylation level of the mgmiRs examined by qMS-PCR in 12 HNSCC cell lines (HNSCC) and 4 head and neck control cell lines (Normal). Red frames highlight mgmiRs with significance difference between HNSCCs and normal (p < 0.05). Figure S3. Selection of mgmiR in HNSCC using HNSCC and control tissues. Relative methylation level of the mgmiRs examined by qMS-PCR in 30 HNSCC tissues (HNSCC) and 25 control tissues (Normal). Red frames highlight mgmiRs with significance difference between HNSCCs and normal (p < 0.05). Figure S4. ROC curves using continuous variables for HNSCC detection. (A). ROC curves comparing the seven mgmiRs with the largest areas under the curve for tissues. (B). ROC curves comparing the seven mgmiRs with the largest areas under the curve for saliva. Figure S5. Variable importance plot from the Random Forest analysis for tissue data including (A) or excluding (B) demographic information. Figure S6. Variable importance plot from the Random Forest analysis for saliva data including (A) or excluding (B) demographic information. (ZIP 509 kb)

补充图S1：头颈部鳞状细胞癌（HNSCC）相关mgmiRs筛选流程。补充图S2：基于头颈部鳞状细胞癌（HNSCC）与对照细胞系的HNSCC相关mgmiRs筛选：通过定量甲基化特异性PCR（qMS-PCR）检测12株HNSCC细胞系（HNSCC组）与4株头颈正常对照细胞系（正常对照组）中mgmiRs的相对甲基化水平，红色框标注出两组间存在显著性差异的mgmiRs（p < 0.05）。补充图S3：基于头颈部鳞状细胞癌（HNSCC）与对照组织的HNSCC相关mgmiRs筛选：通过qMS-PCR检测30份HNSCC组织（HNSCC组）与25份正常对照组织（正常对照组）中mgmiRs的相对甲基化水平，红色框标注出两组间存在显著性差异的mgmiRs（p < 0.05）。补充图S4：用于HNSCC检测的连续变量受试者工作特征曲线（ROC）：(A) 针对组织样本，比较7个曲线下面积最大的mgmiRs的ROC曲线；(B) 针对唾液样本，比较7个曲线下面积最大的mgmiRs的ROC曲线。补充图S5：针对纳入（A）与排除（B）人口统计学信息的组织样本数据集，随机森林分析生成的变量重要性图谱。补充图S6：针对纳入（A）与排除（B）人口统计学信息的唾液样本数据集，随机森林分析生成的变量重要性图谱。（压缩包大小：509 KB）