The gene expression profiling of 3'2'-cCAMP signaling and 2'3'-cGAMP signaling in the BMDMs of WT and STING deficienc mice

3'2'-cCAMP represents a new class of endogenous Cyclic GMP-AMP second messenger. Here, we examined gene expression profiling of 3'2'-cCAMP signaling in the BMDMs of WT and STING deficienc mice Overall design: Total RNA of BMDMs from WT or STING knockout mouse after stimulating with 3'2'-cCAMP or 2'3'-cGAMP was isolated and sequenced by deep sequencing using Illumina HiSeq 2500

3',2'-cCAMP代表一类新型内源性环GMP-AMP第二信使。本研究对野生型（Wild Type, WT）及STING（Stimulator of Interferon Genes）缺陷小鼠的骨髓来源巨噬细胞（Bone Marrow-derived Macrophages, BMDMs）中3',2'-cCAMP信号通路的基因表达谱进行了检测。实验设计概述：分别以3',2'-cCAMP与2',3'-cGAMP刺激野生型或STING基因敲除小鼠的骨髓来源巨噬细胞，提取其总RNA后，采用Illumina HiSeq 2500平台完成深度测序。